One day before the AIPAC conference kicks off in Washington, an anti-Obama pro-Israel group is widening its criticism of President Barack Obama’s record on Israel — while the White House defends its treatment of the relationship.

The trailer for a new 30-minute video, entitled “Daylight: The Story of Obama and Israel,” cuts together clips of Obama quotes and outside commentary to put forth the narrative that Obama has made statements and taken actions as president that have put him out of step with the government of Israeli Prime Minister Benjamin Netanyahu and his supporters.

“We believe that that the borders of Israel and Palestine should be based on the 1967 lines,” Obama is shown saying, a reference to his May, 2011 speech, where he for the first time explicitly defined U.S. policy as supporting the 1967 borders with agreed swaps as the basis for Israeli-Palestinian peace negotiations.

“He didn’t quite have a full grasp of what the full region looks like,” conservative journalist Lee Smith is shown saying in the video. “This is not how you treat an ally.”

The ad goes beyond the Israeli issue to suggest that the president is too solicitous of Muslim concerns. The end of the trailer shows Obama saying, “I want to make sure we end before the call to prayer,” a clip from his town hall meeting with Turkish students in Istanbul in April 2009.

The video was produced by the group the Emergency Committee for Israel, which has spent hundreds of thousands of dollars on its pre-AIPAC publicity campaign, including posters and billboards all over Washington that question Obama’s commitment to prevent Iran from obtaining a nuclear weapon.

“He says a nuclear Iran is unacceptable. Do you believe him?” the posters read. Then, next to a picture of Iranian Supreme Leader Ayatollah Khomeini and President Mahmoud Ahmedinejad, it says, “Do they?”

ECI is run by executive director Noah Pollak and Michael Goldfarb, a former McCain-Palin staffer now working at the consulting firm Orion Strategies and as chairman of the board of the Washington Free Beacon, an new conservative website.

“Obama says a nuclear Iran is unacceptable,” Pollak told The Cable today. “We hope he means what he says, but the recent statements from his administration, his contentious relationship with the Israeli government, and his consistent efforts to weaken congressional sanctions don’t inspire confidence.”

The ECI board is comprised of Weekly Standard Editor Bill Kristol, Gary Bauer, who has endorsed Rick Santorum, and Rachel Abrams, the wife of former NSC official Elliott Abrams, and the author of the controversial Israel-focused blog “Bad Rachel.” The group is also the only Israel-focused advocacy organization to have formed a SuperPAC in the run up to the 2012 election.

As part of its pre-AIPAC activity, ECI took out a full page ad in the New York Times yesterday calling out donors for supporting two liberal advocacy organizations in Washington, the Center for American Progress and Media Matters, and accusing those donors of “funding bigotry and anti-Israel extremism.”

Pollak also said that the video, billboards, and ads happen to refute a pre-AIPAC interview Obama gave to The Atlantic, in which Obama expressed frustration with the attacks coming from conservative lawmakers and groups like ECI that claim he is not pro-Israel.

“Every single commitment I have made to the state of Israel and its security, I have kept,” Obama said. “Why is it that despite me never failing to support Israel on every single problem that they’ve had over the last three years, that there are still questions about that?”

“Obama said today he doesn’t understand why there are questions about his record of support for Israel,” Pollak said. “We think this movie will set the record straight, and remind pro-Israel Americans of the facts of this administration’s failure to stand with Israel at some critical moments.”